Journal: Scientific Reports

Article Title: New insights into tenocyte-immune cell interplay in an in vitro model of inflammation

doi: 10.1038/s41598-017-09875-x

Figure Lengend Snippet: Soluble factors released in co-cultures were similar when performed in transwell assays. Supernatants were evaluated using the Legendplex TM human inflammation panel and the levels of IL-6, IL-8, and MCP-1 are shown for either direct co-culture or transwell assays where tenocytes were cultured in inserts above adherent macrophages ( a ). The mean fluorescence intensity (MFI) is shown for CD80 and HLA-DR on macrophages following direct co culture (co-cultured MΦ) or transwell co-culture (transwell MΦ) with tenocytes ( b ). Supernatants were tested for CICP (C-terminal of type I collagen) using an EIA Kit from tenocytes alone and tenocytes co-cultured with macrophages directly or in transwell assays ( c ). Data are presented as the median with the interquartile range of n = 10 for ( a ) and ( b ) and n = 9 for ( c ), and are considered significantly different when *p < 0.05 with the Kruskal Wallis non-parametric ANOVA with Dunn’s post-test.

Article Snippet: Supernatants were tested for type I Collagen using the MicroVue CICP EIA Kit for determining levels of CICP (C-Terminal of Type I Collagen; Quidel, San Diego, CA, USA) and CICP values were interpolated from the standard curve after measuring the optical density at 405 nm on a microplate reader (Bio-Rad, Munich, Germany).

Techniques: Co-Culture Assay, Cell Culture, Fluorescence